Extended Data Fig. 6: FRTAC-mediated target degradation alleviates tumor cell growth and enhances the T cell-mediated tumor cell killing.
From: Polyvalent folate receptor-targeting chimeras for degradation of membrane proteins
a, Cell impedance assay analysis of the proliferation of H292 cells treated with 50 nM Ctx or 10 nM FR-Ctx (FAR 15). b, Flow cytometry analysis of PBMCs-mediated killing of HCC827 cells treated with PBS, 50 nM Atz or 10 nM FR-Atz (FAR 15) using Annexin V and PI double staining. c,d PBS, 50 nM Atz, or 10 nM FR-Atz (FAR 15)-treatment HCC827 cells were co-incubated with activated PBMCs, the levels of IFN-γ (c) and GzmB (d) in CD8+T cells were examined by flow cytometry. Data in a–d represent three independent experiments, and data are shown as mean ± s.e.m. P values were calculated using an unpaired two-tailed t-tests.
