Extended Data Fig. 4: (related to Fig. 1) KinprolaPKA labeling in response to varying concentrations of PKA modulators in HEK293 cells.
From: Molecular recording of cellular protein kinase activity with chemical labeling
(a) Flow cytometry analysis of HEK293 cells stably expressing cytoplasm-targeted KinprolaPKA labeled with 25 nM CPY-CA for 30 min in the presence of varying concentrations of PKA modulators. (b) Flow cytometry analysis of HEK293 cells stably expressing microtubule-targeted KinprolaPKA treated with increasing concentrations of Iso and CPY-CA for 30 min. Error bars indicate mean ± SEM, data from three (a) or two (b) independent experiments with replicates. Curves were fitted with the sigmoidal function to determine the apparent EC50 values.
