Extended Data Fig. 4: Thioesterase activity of ABHD11 on glutaryl and succinyl esters.
From: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism
a,b, Thioesterase activity of ABHD11-Flag (100 nM) on OGDHc-E2 Lpglu peptide (100 µM) compared to glutaryl-CoA (100 µM) was determined via DTNB assay. Mean ± s.d.; n = 3 independent experiments, one-way ANOVA and Tukey’s post-hoc test. c,d, Thioesterase activity of ABHD11-Flag (100 nM) on succinyl-CoA (100 µM) in the presence or absence of ML226 (2.5 µM) was determined via DTNB assay. Mean ± s.d.; n = 3 independent experiments; one-way ANOVA and Dunnett’s post-hoc test. e, Synthetic Lpsuc OGDHc-E2 peptide. f,g, Thioesterase activity of ABHD11-Flag (100 nM) on OGDHc-E2 Lpsuc peptide (100 µM) in the presence or absence of ML226 (2.5 µM) was determined via DTNB assay. Mean ± s.d.; n = 3 independent experiments; one-way ANOVA and Dunnett’s post hoc test. (g) Column 1 vs column 2: P < 0.0001. h,i, Succinate release from succinyl-CoA (100 µM) (h) or OGDHc-E2 Lpsuc peptide (100 µM) (i) incubated with ABHD11-Flag (100 nM) in the presence or absence of ML226 (2.5 µM) was determined using LC–MS. Mean ± s.d.; n = 2 (h), n = 3 (i) independent experiments; one-way ANOVA and Dunnett’s post-hoc test.
