Extended Data Fig. 7: Effect of ABHD11 inhibition on DNA hydroxymethylation and selected histone marks.
From: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism
a,b, Human CD8+ T cells were isolated from healthy donor peripheral blood mononuclear cells, activated with anti-CD3/CD28 Dynabeads and continuously treated with 1 µM ML226 for 11 days. 5hmC levels were determined using dot blotting of genomic DNA (a), normalized to total DNA staining by methylene blue and adjusted relative to the vehicle condition (b). Each data point represents one donor. Mean ± s.d.; n = 6 donors; paired two-tailed t-test. c,d, Human CD8+ T cells were isolated from healthy donor peripheral blood mononuclear cells, activated with anti-CD3/CD28 Dynabeads and continuously treated with 1 µM ML226 or 1 mM DM-2OG for 11 days. H3K4me3, H3K9me and H3K27me3 levels were determined using immunoblotting (c). Methylation levels were normalized to total histone H3 levels and adjusted relative to the vehicle condition (d). Each data point represents one donor. Mean ± s.d.; n = 4 (ML226), n = 3 (DM-2OG) donors; one-way ANOVA and Dunnett’s post-hoc test.
