Extended Data Fig. 2: Lysine-glutarylation and lipoyl-glutarylation modifications following ABHD11 depletion or following glutarate pertubation.
From: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism
a,b, Quantification of OGDHc-E2 K110 lysine glutarylation (Kglu) in ML226-treated cells from Fig. 1h,i (a), or in ABHD11 KO cells from Fig. 1j (b). Peptide abundance was normalized to the total abundance of modified peptides and adjusted relative to the vehicle or β2M condition. Chromatograms are representative. Mean ± s.d., n = 3 independent experiments. c–e, HeLa cells were treated with 1 mM DEG for 6 h or 24 h. OGDHc-E2 was immunoprecipitated and K110 Lp (c), K110 Lpglu (d), or K110 Kglu (e) were quantified using LC–MS/MS. Control conditions are the same as in Fig. 1h,i and (a). Peptide abundance was normalized to the total abundance of OGDHc-E2 peptides and adjusted relative to the vehicle condition. Chromatograms are representative. Mean ± s.d., n = 3 (e) and n = 4 (c,d) independent experiments; unpaired t-test, two-tailed.
