Extended Data Fig. 5: Cryo-EM data processing for the dynein-Lis1-Nde1 dataset.
From: Nde1 promotes Lis1 binding to full-length autoinhibited human dynein 1
a, A representative cryo-EM micrograph and the flowchart of cryo-EM data processing. b, FSC curve showing the final resolution estimate for the motor domains of the PhiL-Lis1 (2.88 Å) dataset. c, Comparison of Nde1’s effect on Lis1 binding to the open dynein motor. Cryo-EM particle numbers for open dynein-Lis1 and open dynein alone were quantified from the dynein-Lis1 and dynein-Lis1-Nde1 datasets. In the dynein-Lis1 dataset, 493,173 particles correspond to single dynein motors unbound to Lis1, and 624,475 particles correspond to dynein motors bound to Lis1 (including Lis1 dimers and monomers). In the dynein-Lis1-Nde1 dataset, 162,229 particles correspond to dynein motors unbound to Lis1, and 170,995 particles correspond to dynein motors bound to Lis1 (including Lis1 dimers and monomers), indicating that Nde1 does not promote Lis1 binding to the open dynein motor.
