Extended Data Fig. 9: Euphane and tirucallane production resulting from mutations of OSC9.
From: Large-scale mining of plant genomes unlocks the diversity of oxidosqualene cyclases
a, Mean euphane content as a percentage of total euphane and tirucallane content for each OSC product profile. b, Mean Δ7 isomer content as a percentage of total Δ9 and Δ7 isomer content for each OSC product profile. c, Percent change in total accumulation of euphanes and tirucallanes compared to the mean wildtype value (µg/mg). d, Linear regression of % euphane content and the molar volume (mL/mole) of different amino acids at residue 549 (Gly = 36.1, Ala = 53.2, Gln = 86.3, Ile= 100.1, Phe = 113.9, Tyr = 116.2, Trp = 136.7, Values from Zamyatnin, A. A.44 Error bars for all are standard error of the mean (three biological replicates). **** = p ≤ 0.0001, *** = p ≤ 0.001, ** = p ≤ 0.01, * = p ≤ 0.05, ns = p > 0.05) - independent two-tailed T-Tests. Full source data with p values available in Source Data for this figure. See Supplementary Data 4 – Supplementary Database for source Extracted Ion Chromatograms.
