Fig. 6: OSC9 product ratios change with substitution of key residues.
From: Large-scale mining of plant genomes unlocks the diversity of oxidosqualene cyclases
a, Structures of eupha-7,24-dien-3β-ol (11), euphol (5), tirucallol (7) and tirucalla-7,24-diene-3β-ol (18). b, Lowest-energy (affinity = −16.5 kcal mol−1) flexible-residue docking model of the dammarenyl cation with the OSC9 homolog model (Supplementary Data 5, PDB file of the OSC9 homology model; Supplementary Data 6, PDBQT file of the lowest-energy binding model of the dammarenyl cation and OSC9 flexible residues). The position of the C20 cationic carbon and the residues targeted for substitution in this study are highlighted. c, Representative extracted ion chromatograms (EICs, selected ion 411) for the wild-type OSC9 enzyme and mutant variants (scaled to the largest peak) for key substitutions.
