Fig. 4: Engineered cross-feeding and cell–cell adhesion enable controlled growth dynamics in synthetic yeast cocultures.
From: Programmable cell–cell adhesion in synthetic yeast communities for improved bioproduction
a, Diagram of cell growth assay using a microplate reader and the design of cross-feeding cocultures with different surface display architectures. Three auxotrophic systems (Lys–Ade, Trp2–Trp4 and Met–Trp4) were combined with 16 adhesion pairs across three adhesion pair strategies (Strategy 1, Strategy 2.1 and Strategy 2.2). b–d, Growth curves (OD700nm) of the three cross-feeding cocultures over 72 h, each inoculated at a 1:1 initial ratio and incorporating adhesion systems: Lys–Ade (b), Trp2–Trp4 (c) and Met–Trp4 (d). n = 3 biologically independent samples and data are presented as mean ± s.d. Data for other initial ratios (1:10 and 10:1) are shown in Supplementary Figs. 8, 10 and 12. Schematic in a created with BioRender.com.
