Extended Data Fig. 3: Growth dynamics and cell proportions in synthetic yeast cocultures.
From: Programmable cell–cell adhesion in synthetic yeast communities for improved bioproduction
a-c, Growth curves of the cross-feeding coculture pairs and estimated growth of individual population percentages at different initial cell ratios over 72 h (a, lys2Δ–ade8Δ; b, trp2Δ–trp4Δ; c, met14Δ–trp4Δ). Cell 1: lys2Δ (trp2Δ or met14Δ) sfGFP; Cell 2: ade8Δ (or trp4Δ) mRuby2. OD700nm represents the total coculture density. GOD%: The proportion of GFP intensity-converted OD relative to the total OD700nm; ROD%: The proportion of mRuby2 intensity-converted OD relative to the total OD700nm. Data for cocultures with different adhesion pairs are shown in Supplementary Fig. 9, 11, 13. Standard curves for converting fluorescence intensities (GOD and ROD) to OD values are provided in Supplementary Table 2. n = 3 biologically independent samples and data are presented as mean ( ± s.d.). d, Heatmap of maximal OD700nm values within 72 h for each coculture with 16 adhesion pairs, across initial ratios of 1:1, 1:10, and 10:1. n = 3 biologically independent samples and data are presented as mean.
