Fig. 2: AzPico-chromocontrolled electrophysiology of TRPC4.
From: Ideal efficacy photoswitching for chromocontrol of TRPC4/5 channel functions in live tissues
a–g, Electrophysiological whole-cell recordings of TRPC4 currents in voltage clamp mode (a,b,d–g, Vh = −80 mV; c, Vh scan) in HEK293 cells with 10 nM AzPico during photoswitching. a,b, Reproducibility of 36 consecutive photoswitching cycles of 360/440 nm (a, time course; b, overlay of all cycles). c, I/V curves showing that 440 nm drives almost a full return to baseline currents throughout the applied voltage range. d–g, Spectral scans to extract the wavelength dependency of channel current photoswitching on (d,e, cycles of λON/440 nm) and photoswitching off (f,g, 360 nm/λOFF). In e,g, n = 12 biological replicates; data are presented as the mean values ± s.e.m. h–j, Electrophysiology action spectra of AzPico match PSS-based expectations for an efficacy switch (h) but not an affinity switch (i), which is an important distinction, as an affinity switch would have severely concentration-dependent activity (j) (full legend in Supplementary Information).
