Fig. 5: AzHC and AzPico are potent photoswitchable activators of TRPC-dependent Ca2+ responses in mouse hypothalamus.
From: Ideal efficacy photoswitching for chromocontrol of TRPC4/5 channel functions in live tissues
a, Coronal brain slice: cartoon with ARC region in red and microscopy image with Trpc5 immunostained in green and Th+ neurons in red (representative image from n = 4 mice). b–h, Ca2+ responses in Th+ neurons of Th-GCaMP6f (WT) or Th-GCaMP6f-ΔTrpc5 (Trpc5-ko or 5ko) mice. b,c, Single-cell Ca2+ traces before (E) and after (Z) a 355-nm pulse. d,e, ΔAUCλ, light dependency of the AUC as acquired in b,c. f, AzHC only chromocontrols TRPC5-dependent Ca2+ responses; AzPico can chromocontrol Ca2+ responses by another route (likely TRPC4). g,h, mean Ca2+ burst durations and frequencies in WT and 5ko upon E→Z photoswitching. AzHC/AzPico, 500 nM (except in dose–response study); BTDazo, 10 µM. In d–h, each point represents one cell with n cells per group. Box plots show the interquartile ranges, median (line), mean (black rhombus) and s.d. (whiskers). In f–h, statistical analysis was conducted using a Kruskal–Wallis analysis of variance (ANOVA) and Dunn’s P values are shown. In g,h, if no P value is indicated, then the data were not significantly different between the E and Z isomers of AzHC or BTDAzo (P = 0.052–0.999; g, Kruskal–Wallis ANOVA: χ2 (11) = 132.71744, P < 0.0001; h, Kruskal–Wallis ANOVA: χ2 (11) = 142.9224, P < 0.0001). Min–max values and all other details are provided in Supplementary Fig. 22 (full legend in Supplementary Information).
