Fig. 1: Tau phosphorylation induces envelope disassembly.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a, Schematics of the sample preparation of phospho-tau (magenta) and dephospho-tau (cyan). b, MS-determined degree of phosphorylation of phospho-tau (magenta) and dephospho-tau (cyan). Data are presented as the mean ± s.d. (n = 3 replicates; Methods) and displayed at the location of the phosphorylation site along the amino acid sequence of tau (schematic of the sequence shown above the plot). Tau domains are color-coded: N-terminal domains (N1 and N2; gray), proline-rich domains (P1 and P2; blue), microtubule-binding repeats (R1–R4; orange) and domain pseudorepeat (R′, light orange). c, Multichannel fluorescence micrographs of taxol-stabilized microtubules (black; IRM) incubated with 1.5 nM dephospho-tau (cyan; top) or 1.5 nM phospho-tau (magenta; bottom). Scale bars, 2 μm. d, Percentage of taxol-stabilized microtubules covered with tau envelopes after 3 min of tau incubation. Data are presented as the mean ± s.d. (phospho-tau, magenta, n = 14 independent experiments; dephospho-tau, cyan, n = 12 independent experiments). e, Multichannel fluorescence micrographs of 15 nM Bact-tau (red in schematics) after addition of active Cdk5 (top; Bact-tau in magenta) or with heat-deactivated Cdk5 (bottom; Bact-tau in cyan). Microtubules (black) imaged using IRM. Scale bars, 2 μm. f, Time-projected kymograph corresponding to the micrographs from e. Scale bars, 2 μm (vertical) and 1 min (horizontal). g, Normalized difference between the tau envelope coverage before and 10 min after adding active Cdk5 (magenta; n = 6 independent experiments) or deactivated Cdk5 (cyan; n = 8 independent experiments), presented as the mean ± s.d.
