Extended Data Fig. 6: Tau-microtubule interaction is sensitive to the pH of the cell.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a. Fluorescence micrographs of IMCD-3 cells before elevated-pH treatment (top), at t = 0 min after elevated-pH treatment (middle) and t = 9 min after elevated-pH treatment (bottom). Types of cells subjected to the pH treatment: control GFP-tau cells (tau, cyan), GFP-tau-∆N cells (tau-∆N, orange), and GFP-tau + Cdk5/p25 cells (tau-Cdk5, magenta). Left panels show the mScarlet-tubulin signal, right panels the GFP-tau signal. Scale bars: 10μm. b. Density of tau on microtubules before elevated-pH treatment compared to density of tau in the cytoplasm, to give a measure of the binding affinity of tau in the different cells. Data is presented as mean ± s.d. (n = 25 cells for each group in 4 independent experiments). Two-sided t-test p-values (left-to-right): p = 3.26*10-8, 0.0594, 5.89*10-4. c. Mean intensity of tau before elevated pH-treatment, as an indication of the expression level, presented as mean ± s.d. (n = 25 cells for each group in 4 independent experiments). Two-sided t-test p-values (left-to-right): p = 0.0658, 0.8901, 0.0774. Source data for b and c is available with this manuscript. Stars for statistical tests represent: * p < 0.05, *** p < 0.001.
