Extended Data Fig. 9: Tau envelopes protect microtubules against katanin severing.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a. Multichannel fluorescence micrographs (schematics above) of 30 nM phospho-tau (magenta, left) or 5 nM dephospho-tau (cyan, right) showing rapid katanin-GFP-mediated severing of microtubule regions not covered by a tau envelope (panel 2, tau in magenta/cyan, katanin in yellow), followed by much slower disassembly of envelope-covered microtubule regions (panels 3-6). Katanin severing from the boundaries of tau envelopes is indicated by orange arrows; occasional severing within a tau envelope (severing event) is indicated by white arrows. Scale bars: 2 μm. b. Katanin severing events within tau envelope boundaries in phospho-tau envelopes (magenta) and dephospho-tau envelopes (cyan), presented as mean ± s.d. (n = 127, 132 envelopes in 4, 4 experiments). Two-sided t-test, p = 1.85*10-17. c. Comparable tau density within envelopes constituted of 30 nM phospho-tau (magenta) or 5 nM dephospho-tau (cyan), presented as mean ± s.d. (n = 60, 60 envelopes in 3, 3 independent experiments). Two-sided t-test, p = 0.097192. Source data for b and c is available with this manuscript. Stars for statistical tests represent: *** p < 0.001.
