Extended Data Fig. 10: Tau phosphorylation affects envelope functionality in vivo.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a. Relative tau density in cells expressing Katanin-GFP in combination with mCherry-tau ( + tau, cyan) or mCherry-tau-∆N ( + tau-∆N, orange). Tau densities were set to 1 for tau cells and compared to the densities of tau-∆N cells, presented as mean ± s.d. (n = 75, 75 cells in 3, 3 independent experiments). Two-sided t-test, p = 3.3153*10-6. b. Correlation of the relative tubulin density (y-axis) compared to the relative katanin density (x-axis). Correlation coefficients of ‘katanin only‘ is -0.008 (yellow), of ‘+ tau‘ is -0.001 (cyan), and of ‘+ tau-∆N‘ is -0.006 (orange). c. Fluorescence micrographs of IMCD-3 cells co-transfected with mCherry in combination with Cdk5/p25 to visualize transfected cells (‘Cdk5/p25 alone’, left panels) or with mCherry in combination with an empty vector (control, ‘mCherry alone’, right panels). Cells were fixed and stained 12 hours after transfection with anti-tubulin (grey, middle panels) and anti-p25 antibodies (green, bottom panel). Cells expressing mCherry are marked with a yellow dotted line. Scale bars: 5 µm. d. Relative tubulin density in IMCD-3 cells transfected with mCherry in combination with Cdk5/p25 (‘Cdk5/p25 alone’, light pink) or mCherry and empty vector (‘mCherry alone’, grey). Data is presented as mean ± s.d. (n = 63, 67 cells in 2, 2 independent experiments). Two-sided t-test p = 0.46689. e. Relative tau density in cells expressing mCherry-tau (tau, cyan) or mCherry-tau + Cdk5/p25 (tau-Cdk5, magenta). Tau densities were set to 1 for tau cells and compared to the densities of tau-Cdk5 cells (mean ± s.d., n = 75, 75 cells in 3, 3 independent experiments). Two-sided t-test, p = 0.319199. Source data for a, b, d and e is available with this manuscript. Stars for statistical tests represent: *** p < 0.001.
