Fig. 6: Tau phosphorylation affects envelope functionality in vivo.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a, Fluorescence micrographs of IMCD-3 cells expressing katanin–GFP (top), fixed 12 h after transfection and stained for tubulin (bottom). In combination with katanin, cells express full-length mCherry–tau (+tau; cyan), mCherry–tau-∆N (+tau-∆N; orange), mCherry–tau and Cdk5/p25 (+tau-Cdk5; magenta) or Cdk5/p25 in the absence of tau (+Cdk5; light pink). Cells expressing katanin are marked with yellow dotted lines and cells not expressing katanin (nontransfected cells) are marked with white dotted lines. Scale bars, 5 µm. Experiments were performed three times for each cell type, yielding similar results. b, Relative tubulin density 12 h after transfection, presented as the mean ± s.d. (n = 132, 131 and 132 cells in six independent experiments or 60, 60 and 72 cells in three independent experiments). c, Correlation of the relative tubulin density (y axis) compared to the relative katanin density (x axis). Correlation coefficients were −0.49 (katanin only; yellow), −0.25 (+tau; cyan), −0.57 (+tau-Cdk5; magenta) and −0.54 (+Cdk5; light pink).
