Extended Data Fig. 1: Tau phosphorylation reduces tau envelope propensity.
From: Tau phosphorylation impedes functionality of protective tau envelopes
a. Mass-spectrometry-determined degree of phosphorylation of phospho-tau (magenta) and dephospho-tau (cyan), presented as mean ± s.d. (n = 3, 3 independent samples). Phosphorylation sites with no peptide coverage are marked with a black cross. The color-coded legend corresponds to the colorscheme in Fig. 1b. b. Total relative intensities measured for all peptides covering a phosphorylation site, corresponding to data in panel a and Fig. 1b, presented as mean ± s.d. (n = 3, 3 independent samples). The color-coded legend corresponds to the colorscheme in Fig. 1b. c. Western blot of phospho-tau (insect cell expressed tau, magenta) and dephospho-tau (phosphatase-treated insect cell expressed tau, cyan) using total tau antibody (tau-5, top panel) and phospho-specific antibody (pS404, bottom panel). Three independent samples per condition were analyzed from one Western blot. d. Total tau levels (tau-5 intensity) of phospho-tau (magenta) and dephospho-tau (cyan) samples, presented as mean ± s.d. (n = 3, 3 independent samples). e. Quantification of relative phosphorylation levels, normalized to total tau. Mean phospho-tau (magenta) was set to 1 and compared dephospho-tau levels (cyan), presented as mean ± s.d. (n = 3, 3 independent samples). f. Fluorescence micrographs of 10 nM phospho-tau (left, phospho-tau in magenta) or 10 nM dephospho-tau (right, dephospho-tau in cyan) on taxol-stabilized microtubules. Linescans of the indicated microtubules (yellow dotted line) are shown below the micrograph. The grey dotted line indicates the half-maximum intensity threshold used to define tau envelope boundaries. Scale bars: 2 μm. Source data for a, b, c, d, e and f is available with this manuscript.
