Fig. 1: Engineering a thermoswitchable E. coli transcription factor by AsLOV2 domain insertion.
From: Modular engineering of thermoresponsive allosteric proteins
a, Assay schematics. b,c, E. coli transformed with plasmids encoding the indicated AraC–LOV variants and an mRFP reporter were induced with 400 µM IPTG and 16 mM arabinose and incubated at 37 °C or 41 °C for 16 h followed by measurements of reporter fluorescence (RFP) and culture density (OD600) in a plate reader (b) or flow cytometry (c). d, Close-up views of the AsLOV2 crystal structure (PDB 2V0U). Substituted residues are marked in red and functionally critical elements are marked in blue. Hydrogen bonds are indicated. In b, data represent the mean ± s.d. (n = 3 independent experiments). Statistical analysis was conducted using a two-sided Student’s t-test. **P < 0.01 and ****P < 0.0001. WT, wild-type.
