Extended Data Fig. 7: Flow cytometry analysis and cytokine response of monocytes from healthy and RA or MS patient donors.
From: A druggable redox switch on SHP1 controls macrophage inflammation
a, Gating strategy for flow cytometry analysis of healthy donors or RA and MS patients PBMCs isolated monocytes using CD45, CD14 and CD16 staining (n = 3 for each healthy and patient group). b, Percentage of CD45 + CD14 + CD16- monocyte population of interest (n = 3 for each healthy and patient group). c, Pro-inflammatory cytokine TNF, IL-6 and IL-1β levels in cell supernatants of healthy donors and RA and MS patients monocytes pre-incubated 3 h with SCA1, SCA9, SCA7 and SCA25 (20 μM) followed by 6 h LPS stimulation (100 ng/ml) (n = 3 for each healthy and patient group). For measurement of pro-inflammatory cytokine IL-1β levels in cell supernatants, cells were additionally treated with adenosine triphosphate (ATP, 5 mM) for 45 min following LPS treatment. Data are mean ± s.e.m. P values calculated using two-tailed Student’s t-test for unpaired comparison or two-way ANOVA for multiple comparisons.
