Extended Data Fig. 1: Redox sensitivity of SHP1 Cys102 and selectivity of engagement by SCA1 in macrophage cysteine proteome.
From: A druggable redox switch on SHP1 controls macrophage inflammation
a, Schematic of Oximouse/OxImmune workflow for quantification of cysteine redox state, illustrating CPT labeling, TMT multiplexing, IMAC enrichment, and MS analysis. Created in BioRender. Ng, M. (2025) https://BioRender.com/e74b682. b, Relative gene expression of differentiation markers in THP-1 monocytes treated with either 10 ng/ml PMA for 24 h or 100 ng/ml PMA for 48 h (n = 4 biological replicates). c, Relative cysteine oxidation level of SHP1 Cys102 in THP-1 MDMs treated with N-acetylcysteine (NAC, 10 mM), cell permeable TCEP (tmTCEP, 1 mM) (n = 5) or lipopolysaccharide (LPS, 100 ng/ml) (n = 3) for 1 h. d, Relative cysteine oxidation level of SHP1 Cys102 in THP-1 MDMs (n = 3) treated with LPS (100 ng/ml) for the indicated time periods. e, Pairwise comparisons of R for each cysteine quantified in iBMDM replicates treated with 5, 10, 20, or 40 μM SCA1 for 3 h (37 °C), followed by CPT enrichment. R = (S/N of DMSO) / (S/N of SCA1), where S/N is signal-to-noise ratio. f, SHP1 Cys102 site-specific percent modification by SCA1 in iBMDMs at the indicated concentrations. Data are mean ± s.d. of n = 2 biological replicates. g,h, SHP1 (g) or SHP2 (h) cysteines site-specific percent modification by SCA1 in iBMDMs (n = 2 biological replicates) at the indicated concentrations. Data are mean ± s.e.m. (in b) or s.d. (in c-h). P values calculated using one-way or two-way ANOVA for multiple comparisons.
