Supplementary Figure 4: Flow cytometry gating strategy and longitudinal levels of nasal T cells and monocytes.
From: Inflammation induced by influenza virus impairs human innate immune control of pneumococcus
(a), Gating strategy of nasal cells for one representative volunteer. (b), Numbers of T cells at the nasal mucosa. Median and interquartile range are shown for cell numbers normalized to numbers of epithelial cells for control carriage+ (n = 24), control carriage– (n = 37), LAIV carriage+ (n = 25) and LAIV carriage– (n = 30) groups. The x axis shows days relative to inoculation. (c), Median and interquartile range of monocyte numbers in the control group over time with carriage detected by classical microbiology (carriage+, n = 24), molecular methods only (lytA+, n = 16) or not at all (carriage–, n = 22). *P = 0.038 at day 2 and P = 0.030 at day 29, **P = 0.002 by two-tailed Wilcoxon paired non-parametric test.
