Supplementary Figure 4: Increased expression of co-inhibitory receptors on SFR-KO iNKT cells.
From: SLAM receptors foster iNKT cell development by reducing TCR signal strength after positive selection
(a-d) Flow cytometry analyses showing expression of PD-L1 (a, c) and PD-L2 (b, d) on total, NK1.1- or NK1.1+ thymic and splenic iNKT cells (a, b) and CD4+CD8+ (double-positive; DP) thymocytes (c, d) from WT and SFR KO mice. n = 5 (a-d). Numbers in histograms (a, c) or plots (b, d) indicate the MFIs (a, c) and percentages of iNKT cells (b) or DP thymocytes (d) expressing the indicated marker. (e, f) Flow cytometry analyses showing CD160 (e) and CTLA-4 (f) expression on thymic and splenic iNKT cells from WT and SFR KO mice. n = 6 (e). (g) A representative experiment of the data depicted in Fig. 4e. In (a-e), a representative experiment is shown on the left, while data from multiple mice are depicted on the right. Each symbol (a-e) represents an individual mouse; error bars represent mean with s.d.. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns P > 0.05 (two-sided unpaired t-test). Data are representative of 2 (a-e); 3 (f,g) independent experiments.
