Supplementary Figure 6: iNKT cell development in mice with single or triple deficiency in SLAM family receptors.
From: SLAM receptors foster iNKT cell development by reducing TCR signal strength after positive selection
(a, b) Flow cytometry analyses showing expression of SLAM family receptors on various stages of thymic (a) and splenic (b) iNKT cells from WT mice. (c-e) Flow cytometry analyses showing iNKT cells from thymus and spleen of single SFR (c, d) and triple SLAMF1-SLAMF5-SLAMF6 (e) KO mice, in the indicated genetic background, 129S1 or C57BL/6 (B6). Graphic representations of the numbers (c,e), frequencies (c,e) and PD-1 expression (d) on iNKT cells. n = 7 (SLAMF1 KO and 2B4 KO), 5 (Ly-9 KO, CD84 KO and SLAMF7 KO), 6 (SLAMF6 KO) (c, d); n = 8 (e). Each symbol (c-e) represents an individual mouse; error bars represent mean with s.d.. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001, ns P > 0.05 (two-sided unpaired t-test). Data are representative of 2 (a, b); 3 (SLAMF1 KO, 2B4 KO, CD84 KO and SLAMF7 KO), 4 (Ly-9 KO) and 5 (SLAMF6 KO) (c, d) and 4 (e) independent experiments.
