Extended Data Fig. 6: Protective efficacy of MutB/C-based vaccine against ZIKV infection at 3 DPI.
a-c, Groups of Ifnar1−/− mice (n = 4) (Female:male of 2:2 in both WT and MutB groups; 3:1 in MutC group; 4:0 in sham group) received a single immunization of 1.6 × 1011 vp of AdC7-M/E-WT, AdC7-M/E-MutB, AdC7-M/E-MutC via the i.m. route. PBS was given as the sham vaccine. Serum samples were collected for detection of ZIKV-E-specific IgG (a) and NAb titers (b). Thirty-days after vaccination, mice were challenged with 5 × 106 FFU of ZIKV-SMGC-1 via the i.p. route. At 3 DPI, tissues from the brain, spinal cord, testis, spleen, liver and eye were harvested for measurement of ZIKV loads (c). Data are means ± SEM. The dashed line indicates the LOD.
