Extended Data Fig. 1: Antibodies induced by ZIKV vaccines enhance DENV infections.
a, Neutralizing activities of ZIKV vaccines. 6-8-week old female BALB/c mice (n = 5 biologically independent samples) were immunized with a single dose of AdC7-M/E or AdC7-prM/E (1.6 × 1011 vp) via i.m. route. PBS was given as a sham vaccine. Sera were collected from blood at 4 weeks postvaccination. Magnitudes of NAbs were measured as MN50 titers. Data are means ±standard errors of means (SEM). P values were analyzed with One-way ANOVA test (ns, not significant). The dashed line indicates the LOD. b-e, ADE activities of the immune sera (n = 3 biologically independent samples). Enhancement curves of K562 cells infected with DENV1 (b), DENV2 (c), DENV3 (d), and DENV4 (e) in the presence of serially diluted mouse immune sera as indicated. Infected cells were quantified by flow cytometry and were normalized to the peak infectivity of FLE mAb Z6. Data are means ± SEM. f, ADE activities of the human mAb Z6. Enhancement curves of K562 cells infected with DENV1, 2, 3 and 4 in the presence of serially diluted Z6 as indicated.
