Extended Data Fig. 5: Additional images of Myc expression within GCs.
a, Cd23Cre/+ and b, Bmi1f/fCd23Cre/+ mice were infected with LCMV-Docile and GCs assessed at d7 post-infection as detailed in Fig. 4. Panels show individual staining (IgD, CD3, PNA and Myc; combined top row). Representative images of n = 4 mice per genotype from two individual experiments. Scale bar = 100μm. c, Number of unique synonymous and non-synonymous mutations per clone in CDR and FW regions of GC B cell VH gene repertoire. GC B cells were isolated from Cd23Cre/+ and Bmi1f/fCd23Cre/+ mice infected with either LCMV-WE or LCMV-Docile, d14 post-infection. d, Log ratio of non-synonymous to synonymous mutations in total CDR and total FW regions; median values shown. The boxplots (c, d) visualize the median (middle hinge), two hinges (first and third quartiles, 25th and 75th, respectively), two whiskers (values no larger than the inter-quartile range, 1.5*IQ), and all individual outlying points. e-f, Selection pressure in VH CDR and FW regions; (e) shows individual mice, (f) selection pressure of pooled mice per genotype and infection.
