Extended Data Fig. 1: Worse stroke outcome in aged male and female mice.
a, mNSS scores of young and aged male mice up to 7 days poststroke (n = 10 young, n = 6 aged, Mixed- effect Model, with Bonferroni post-hoc, one experiment, *p ≤ 0.05, **p ≤ 0.01). b, Kaplan-Meier survival curve showing mortality up to 8 days poststroke (n = 10 young and n = 8 aged male mice, Mantel-Cox test, *p = 0.0341). c- e, Stroke in young (2–4 months) and aged (18–24 –months) female mice. c, mNSS of young (n = 7) and aged (n = 4) female mice measured with the mNSS at 24h poststroke (2-tailed, Mann-Whitney test, **p = 0.003) and Kaplan-Meier survival curve up to 3days poststroke (n = 7 young, n = 5 aged female mice; Mantel-Cox test, **p = 0.0053). d, Staining for neurons (NeuN+ in brown) at 3 days poststroke and quantification of neurons (NeuN+) in the peri-lesional area (n = 7 young, n = 5 aged female mice, ***p = 0.001). e, Quantification of ischemic volume by Cresyl Violet staining at 3 days poststroke (n = 7 young n = 8 aged female mice). f-i, Representative images, and density of DHE+ (in red) astrocytes (GFAP+, green in f), neurons (NeuN+, green in g), blood vessels (CD31+, green in h) and pericytes (PDGFRb+, green in i) in the peri-lesional area at 24h poststroke in male young and aged mice (n = 3 mice per group *p = 0.0358). Cell nuclei are stained in blue (DAPI). j, Representative confocal microscopy images of 3NT+ cells (in green) in the ischemic cortex, and density of 3NT+ cells in the ischemic area of young and aged mice at 3 days poststroke (n = 8 young, n = 4 aged mice, **p = 0.0094). In the scatterplots each dot corresponds to a single mouse; mean ± SEM is shown. Scale bar in d, f, g, h, i, j, 50µm. 2-tailed t-test if not otherwise indicated.
