Extended Data Fig. 7: TRMs maintain tissue integrity during pancreatitis.

a. Flow cytometry of pancreas F4/80⁺MHCII^hi/lo macrophages after treatment with CSF1Ab+CLD or IgG+PBS, then a 10-day recovery period (day -1), then cerulein (cer) treatment by 6 hourly i.p. injections every other day for one week (day 7). b. Density of MHCII^hi pancreas macrophages from mice treated as in a; day -1, n = 4mice/group; day 7, n = 3mice/group. c. Density of MHCII^lo pancreas macrophages from mice treated as in a; day -1, n = 4mice/group; day 7, n = 3mice/group, left to right *P = 0.0002 and *P = 0.0002. d. Kaplan-Meier survival curve and body weight measurement of C57BL/6 mice treated with IgG+PBS or CSF1Ab+CLD as in a, followed by cerulein-loaded osmotic pump (10 µg/day cerulein) implantation; IgG+PBS, n = 10mice; CSF1 Ab+CLD, n = 6mice, *P < 0.0001 in survival analysis. e. Relative pancreas weight in C57BL/6 mice treated as in d; IgG+PBS, n = 10mice, CSF1Ab+CLD, n = 4mice, *P < 0.0020. f. IHC images of H&E and amylase stain in pancreas from mice implanted with DMSO-loaded osmotic pump, cerulein treatment as in a, or cerulein-loaded osmotic pump, scale bars, 100 µM. g. Blood glucose concentration at humane survival endpoint in mice treated as in d; IgG+PBS, n = 7mice; CSF1Ab+CLD, n = 6mice, *P = 0.0012. h. Serum amylase level in mice treated as in f; left to right n = 4mice, n = 6mice, and n = 6mice, and *P = 0.0381 and *P = 0.0390. i. H&E-stained liver from mice treated as in f, scale bars, 100 µM. j. Blood Ly6C^lo and Ly6C^hi monocytes, and pancreas F4/80⁺, F4/80⁺MHCII^hi, and F4/80⁺MHCII^lo macrophages, and flow cytometry of F4/80⁺MHCII^hi/lo pancreas macrophages in CCR2-WT and CCR2-KO mice treated with cerulein by i.p. injection as in a; n = 4 mice/group and *P = 0.0286 in blood Ly6C^lo and Ly6C^hi analysis, n = 7 mice in veh CCR2-WT group, n = 5 mice in veh CCR2-KO group, n = 8 mice in cer CCR2-WT group, and n = 6 in cer CCR2-KO group in F4/80⁺, F4/80⁺MHCII^hi, and F4/80⁺MHCII^lo analyses, and *P = 0.0047 in F4/80⁺MHCII^hi macrophage analysis. k. Kaplan-Meier survival curve and body weight measurement of CCR2-WT and CCR2-KO mice implanted with cerulein-loaded osmotic pumps, as in d; n = 8 mice in CCR2-WT group, n = 10 mice in CCR2-KO group. Data are presented as mean ± SEM unless otherwise indicated. n.s., not significant; *p < 0.05. For comparisons between two groups, Student’s two-tailed t-test was used.

Source data