Extended Data Fig. 9: A schematic illustration of the second-generation CAR.
From: A second-generation M1-polarized CAR macrophage with antitumor efficacy
All the CARs comprise an extracellular scFv domain recognizing a specific epitope, a transmembrane (TM) domain and a hinge region from CD8α. The truncated CAR was designed as the negative control without the intracellular signaling domain. The CD3ζ-CAR containing a single intracellular CD3ζ signaling domain was defined as the first-generation CAR corresponding to the first-generation T-CAR. The TIR-CAR contains an intracellular TIR signal transduction domain. The CD3ζ-TIR-CAR containing CD3ζ and TIR in the intracellular domain was defined as the second-generation CAR and possesses higher proinflammatory activity than the first-generation CAR upon binding to a specific antigen.
