Extended Data Fig. 8: Gating strategies and flow cytometry plots for the studies in mice to assess impact of environmental triggers on macrophage phenotype.
(a) Gating strategy used for identification of mouse LCM populations based on LYVE1 and CD206 expression. (b) Representative plots of Fig. 5b showing expression of CD206 and LYVE1 by LCMs from control (Gata6fl/fl) and Csf1rERCre x Gata6fl/fl mice 16 days post-tamoxifen administration. (c) Body weight from female mice fed either normal chow diet (n = 6) or high-fat diet (n = 5) for 5 months to induce obesity. Data representative of two similar experiments. A two-sided Mann-Whitney test was used for statistical analysis (p = 0.0087). ** represents p < 0.01 and data are represented as mean value +/− SEM. (d) Isotype controls for CD206 and LYVE1, comparing to the expression of control or high-fat diet mice. (e) Gating strategy used for analysis of LCMs from wild-caught mice.
