Extended Data Fig. 3: Further analysis of peritoneal MNPs between mouse and human.
(a) Heatmap of differentially expressed genes (rows) of different clusters (columns). Heatmap colors indicate Z-transformed expression of genes in each row, with scale depicted in legend. Annotations (left) highlight representative genes with high differential gene expression within each cluster, relative to other clusters. Colors of gene names indicate corresponding clusters in Fig. 2a. (b) Dot plot showing the average Z-transformed normalized expression of markers for large cavity-, converting- or monocyte like macrophages. The size of each dot indicates the fraction of cells expressing each gene; the color scale represents Z-transformed normalized expression. (c) The projection of cells from each cluster in Fig. 1 in the new UMAP space of Fig. 2. Colors correspond to Fig. 1a. (d) UMAP projection showing the different cell cycle phases of the peritoneal immune cell subsets in mouse or human separately. Colors representing the predicted classification of cell cycle phase based on the S and G2/M scores calculated by the CellCycleScoring function in the Seurat package. (e) The proportions of cells in each cell cycle phase of mouse and human (n = 7 human adult samples and n = 3 mouse samples). Bars depict means with error bars representing standard deviation. * represents p < 0.05, two-tailed t-test.
