Extended Data Fig. 7: Interactions of Aiolos with STAT5 and RUNX members.
From: The transcription factor Aiolos restrains the activation of intestinal intraepithelial lymphocytes
a, A pie chart showing the chromosomal location of Aiolos binding regions in the indicated cell types. b, A Venn diagram depicting Aiolos binding peaks in γδ IELs and DN IELs. c, Represenative histogram plots and quantification of pSTAT5 expression in γδ IEL, DN IEL from the small intestine of Ikzf3+/+ and Ikzf3−/− mice. Cells were either not stimulated or stimulated for 15 minutes with IL-15 (100 ng/ml). d, The results of permutation test designed to analyze the significance of overlap of Aiolos with either STAT5, RUNX1 or RUNX3. Evperm: the number of permutation events, Evobs: the number of observed events (overlapped peak counts). Data are representative of two independent experiments and all samples are biological replicates (n = 4 in c). P values were calculated by unpaired, two-tailed Student’s t-test in c and Permutation test in d (ChIPpeakAnno). Data represent mean ± SD. pSTAT5 = phosphorylated STAT5, UTR = untranslated region.
