Fig. 4: Aiolos-deficient IELs are hyperresponsive to IL-15.
From: The transcription factor Aiolos restrains the activation of intestinal intraepithelial lymphocytes
a, Representative flow cytometry plots showing CD122 and TCF1 expression in γδ uIELs and DN uIELs isolated from the small intestine epithelium of Ikzf3+/+ mice (n = 5). b, Flow cytometry plots and quantification of NK1.1+ γδ uIELs and NK1.1+ DN uIELs in the small intestine epithelium of Ikzf3+/+ and Ikzf3−/− mice (n = 5). c, Flow cytometry plots and quantification of NK1.1+ γδ uIELs and NK1.1+ DN uIELs in the duodenum, jejunum and ileum of Ikzf3+/+ and Ikzf3−/− mice (n = 5). d, Flow cytometry plots and quantification of NK1.1+ γδ uIELs and NK1.1+ DN uIELs isolated from the small intestine epithelium of Ikzf3+/+ and Ikzf3−/− mice 72 h after in vitro culture with 10 ng ml−1 or 100 ng ml−1 of IL-15. a–d, Data are representative of two independent experiments and all samples are biological replicates. Data represent the mean ± s.d. P values were determined by one-way ANOVA with Tukey’s multiple-comparisons test in b and c, and by two-way ANOVA with Tukey’s multiple-comparisons test in d.
