Extended Data Fig. 3: Suboptimal TCR stimulation results in a heightened decline in intratumoral Tpex.
From: Antitumor progenitor exhausted CD8+ T cells are sustained by TCR engagement
a–g Related to Fig. 1a. a, Representative flow plot of P14 percentages in tumors, related to Fig. 2a. b,c, Percentages of Ki67+ (b, n = 4–7) and Gzmb+ (c, n = 3–7) P14s in tumors. d, GMFI of PD1 in tumors, n = 3–5. b–d Gated on P14s, representative data of more than two independent experiments. Multiple two-tailed unpaired t test (mean ± s.e.m.), FDR = 0.05, q value indicated in each graph. e,f,h, GMFI and percentages of CX3CR1+ P14s in tumors, n = 5 (e,h), n = 6–8 (f). P value 0.0434 (e, left) and 0.0198 (h, left). g, GMFI of GFP. Gated on P14-Nur77-GFP CD8+ T cells in LLC-M9 tumors, n = 5 per group. e–h, Representative data of two independent experiments. i, Representative flow plot of T cell percentage in peripheral blood with PBS (Ctr) or FTY720 treatment. j,k, Percentages of P14s in tumors (j, n = 8–13) and tdLN (k, n = 11–13), related to Fig. 2d. l, Percentages of P14s in tdLN, related to Fig. 2fn = 4. m, Percentages and absolute cell numbers of P14s in tdLN, related to Fig. 3a, n(M9) = 9, n(C6M9) = 10. P value 0.0254 (left) and 0.0216 (right). n, Percentages of CX3CR1+ P14s in tumors, related to Fig. 3d. Gated on Tim3+ P14s. n(M9) = 8, n(C6M9) = 6. j–n, Representative data of three independent experiments. e–h,j–n, Two-tailed Student’s t test (mean ± s.e.m.). P value * < 0.05, **** < 0.0001, ns represents not significant. n represents numbers of mice per group.
