Extended Data Fig. 6: Reporter mouse lines for BACH2 and BLIMP-1 induction in MBCs.

a, Schematic diagram of the BRISK^BACH2 and BRISK^BLIMP-1 mouse systems. Col1a1^{Teton-Bach2 or Teton-Blimp-1} alleles were generated by knocking-in the expression cassette into the Col1a1 safe locus with CRISPR/Cas9. BRISK^BACH2 and BRISK^BLIMP-1 mice were obtained by further breeding with Cd79^Cre and Rosa26^LSL-rtTA3 mice. b, The experimental design. c-e, Gating strategies for identifying NP-binding MBCs (c), IgG⁺ or non-IgG MBCs (d), PDL2⁺CD80⁺ DP or PDL2^-CD80^- DN MBCs (e). f, Representative FACS plots showing gates for the uninduced and induced GFP⁺ B1-8^hi cells from donor mice of a BRISK^BACH2 genotype. GFP⁺ B1-8^hi cells from donor mice of a CD79^Cre;Rosa26^LSL-rtTA3 genotype without the tet-inducible apparatus were used as control. g, Summary data of secondary PCs or GCs derived from uninduced IgG⁺, induced IgG⁺, uninduced non-IgG, and induced non-IgG B1-8^hi MBCs of a BRISK^BACH2 genotype. h, Summary data of secondary PCs or GCs derived from uninduced IgG⁺, induced IgG⁺, uninduced non-IgG, and induced non-IgG B1-8^hi MBCs of a BRISK^BLIMP-1 genotype. i, Summary data of secondary PCs or GCs derived from uninduced DP (PDL2⁺CD80⁺), induced DP, uninduced DN (PDL2^-CD80^-), and induced DN B1-8^hi MBCs of a BRISK^BACH2 genotype. j, Summary data of secondary PCs or GCs derived from uninduced DP (PDL2⁺CD80⁺), induced DP, uninduced DN (PDL2^-CD80^-), and induced DN B1-8^hi MBCs of a BRISK^BLIMP-1 genotype. g-j, Data pooled from 3 independent experiments. Each symbol indicates one mouse (n=11 for IgG⁺, DP and DN; n=10 for non-IgG), and lines denoting means. P-values by two-sided unpaired student t tests.

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