Extended Data Fig. 4: Functional enrichment analyses of M-DEGs in peripheral immune cell subsets.
a. Bar plot showing the numbers of total M-DEGs defined by scRNA-seq in each cell subset. b. Bar plot showing the numbers of upregulated (left panel) and downregulated (right panel) M-DEGs in each cell subset. c. Heatmap showing the functional enrichment analyses of upregulated M-DEGs in different peripheral immune cell subsets. d. Heatmap showing the functional enrichment analyses of downregulated M-DEGs in different peripheral immune cell subsets. e. Functional enrichment network of the common M-DEGs in Fig. 2c. f. Functional enrichment network of the common M-DEGs in Fig. 2d. g, h. The DEGs were classified into distinct clusters by Mfuzz based on the temporal trajectories of their expression. The DEG clusters whose expression peaked in children (144 genes, g) and the elderly (90 genes, h) are shown. i. Heatmap showing the functional enrichment analyses of the genes whose expression peaked in children in (g). j. Heatmap showing the functional enrichment analyses of the genes whose expression peaked in the elderly in (h). k. The positive correlation of LGALS1 expression with age and the negative correlation of CCR7 and TMIGD2 expression with age via bulk RNA-seq analyses (n = 34 samples). The blue line indicates linear regression. The gray shadow covers the 95% confidence interval. Spearman correlation coefficient ρ and P values are indicated. A P value < 0.05 was considered statistically significant. P values were determined by hypergeometric test in c–f,i and j. DEG, differentially-expressed genes; M-DEGs, monotonically differentially expressed genes.
