Extended Data Fig. 6: Characteristics of T cell subsets across the entire lifespan.
a. Bar plot showing the percentage of TCR detection in each αβ T cell subset and in total αβ T cells. b. Bar plot showing the frequency of CDR3 amino acid length from TCR α-chains (top panel) and TCR β-chains (bottom panel) across eight life stages. Error bar shows the mean ± SEM. c. Box plots showing the clonal expansion levels of each αβ T cell subset quantified by STARTRAC-expa metric in αβ T cell subsets across 13 age groups. d. Box plots showing TCR repertoire diversity in CD4+ naive T cells (CD4_Naive_CCR7; left panel), CD8+ naive T cells (CD8_Naive_LEF1; middle panel) and MAIT cells (CD8_MAIT_SLC4A10; right panel) across eight life stages. The diversity of TCR repertoires was measured by Chao1 method. e. Box plots showing TCR repertoire diversity in CD8_MAIT_SLC4A10 cells across eight life stages. The diversity of TCR repertoires was measured by Inverse Simpson Index. f. Representative gene interaction network of upregulated genes in (Fig. 5k) using the STRING database (https://string-db.org/). Edge thickness represents the strength of data support from the STRING database. g. Box plots showing normalized protein expression of CD161 (encoded by KLRB1) in CD8_MAIT cells in children (1–6 years old), adolescents (12–18 years old), adults (30–60 years old), and the elderly (70–90 years old) based on the CyTOF analyses. In box plots c–e and g, median (center line), first and third quartiles (box), and extra 1.5× interquartile ranges (whiskers) are shown, and the black line indicates LOESS regression. CDR3, complementarity-determining region-3.
