Fig. 2: Global transcriptome dynamics of peripheral immune cells throughout the human lifespan.
a, Bar plot showing the number of DEGs derived from scRNA-seq analyses of all cell subsets. b, Heatmap showing the functional enrichment analyses of DEGs in all cell subsets. P values were determined by hypergeometric test. c, Bar plots showing the frequencies of upregulated and downregulated M-DEGs derived from scRNA-seq analyses observed in all cell subsets. All M-DEGs with a frequency of ≥5 are displayed. d, Line plots showing the expression trajectories of upregulated (left panel) and downregulated (right panel) age-related genes identified by bulk RNA-seq analyses. Data are shown in terms of gene expression z-score. Each gray line represents the expression trajectory of a gene. e, Venn diagrams showing the number of overlapping genes between common M-DEGs identified in scRNA-seq data and age-related genes identified in bulk RNA-seq data. Among the 56 common M-DEG genes defined by scRNA-seq, 13 intersected with the age-related genes defined by bulk RNA-seq. f, Heatmap showing the expression of SELL gene in seven cell subsets by scRNA-seq and CD62L protein (encoded by SELL) in corresponding cell subsets by CyTOF among eight life stages.
