Extended Data Fig. 4: The N3 neutrophil subset predicts poor survival.
From: Microbiota-shaped neutrophil senescence regulates sexual dimorphism in bladder cancer
(a, c) Prognostic value of specific human N3 (a) and mouse N3 (c) enriched genes for overall survival in bladder cancer patients from TCGA datasets. Forest plots showing HR and 95% CI derived from univariate Cox analysis in male and female bladder cancer patients. Within the forest plot, HR for each variable is depicted as a box, and 95% CIs are shown as horizontal. The vertical line crossing the value of 1.0 represents a non-statistically significant effect, and odds greater than one indicate worse effects. (b, d) Distribution of risk score, survival time, specific human N3 (b) and mouse N3 (d) enriched genes involved in the prognostic model among bladder cancer patients in TCGA datasets. (e) QPCR analysis of mRNA expression of the genes including Anxa2, Rdh11, S100a8, St3gd16, Taldo1 and Timp2 in tumor-infiltrated senescence-like fluorescein di-β-D-galactopyranoside (FDG)+ and non-senescence FDG− neutrophils (n = 3). Bar graphs are presented as mean ± s.e.m. A two-tailed Student’s t-test was performed for comparisons. The data are representative of at least three independent experiments.
