Extended Data Fig. 5: Immune profiling in DlstTreg-WT and DlstTreg-KO mice.
From: Succinate drives gut inflammation by promoting FOXP3 degradation through a molecular switch
a-c, Representative flow cytometry (a) and quantification of the percentage of CD62LhiCD44low naïve and CD62LlowCD44hi effector CD4+ T cells in the spleen (b) and lymph node (c) from 3-week-old DlstTreg-WT and DlstTreg-KO mice. n = 7, per group. d-f, Representative flow cytometry (d) and quantification of the percentage of CD62LhiCD44low naïve and CD62LlowCD44hi effector CD8+ T cells in the spleen (e) and lymph nodes (f) from 3-week-old DlstTreg-WT and DlstTreg-KO mice. n = 7, per group. g-h, Representative flow cytometry (g) and quantification (h) of the percentage of IL-2+CD4+FoxP3-, IL-4+CD4+FoxP3-, IL-17+CD4+FoxP3-, and IFN-γ+CD4+FoxP3- T cells in the spleen from 3-week-old DlstTreg-WT and DlstTreg-KO mice. n = 6, per group. i–n, Histogram and quantification of specified cell surface proteins in CD4+CD25+FoxP3+ Treg cells in the thymus (i,j), spleen (k,l), and lymph node (m,n) from 3-week-old DlstTreg-WT and DlstTreg-KO mice. n = 6, per group. Numbers in quadrants indicate the value of mean fluorescent intensity (MFI) (i,k,m). Data are shown as means ± s.d. (b,c,e,f,h) or sem (j,l,n), and analyzed by two-tailed unpaired Student’s t test.
