Extended Data Fig. 1: Analysis of succinate on DSS-induced colitis.
From: Succinate drives gut inflammation by promoting FOXP3 degradation through a molecular switch
a, The absolute number of CD45+ immune cells in the colon at day 7 in C57BL/6 J mice treated with 2.5% DSS (hereafter 2.5% DSS, n = 8) or 3% DSS (3% DSS, n = 5) in drinking water for 7 days, or with 3% succinate in drinking water for 3 days before treatment with 2.5% DSS and 3% succinate for 7 days (2.5% DSS + 3% succinate, n = 8) or none of the above (control, n = 4). b, Representative flow cytometry of CD4+ and CD8+ T cells in CD45+ cells in the inflamed colon at day 7 in mice as in a. c, The percentage and absolute number of CD4+CD45+ T cells in the inflamed colon at day 7 in mice as in a. d, The percentage and absolute number of CD8+CD45+ T cells in the inflamed colon at day 7 in mice as in a. e-f, Representative flow cytometry (e) of and absolute number (f) of CD45+CD4+FoxP3- conventional T cells in the inflamed colon at day 7 in mice as in a. Data are shown as means ± s.d., and analyzed by one-way ANOVA with Tukey’s post hoc test. Numbers in the bars represent exact P values. Data are representative of two independent experiments.
