Extended Data Fig. 5: NR and HKL restore the effector function of PBMC-derived NK cells.
From: Targeting lactylation reinforces NK cell cytotoxicity within the tumor microenvironment
(a-b) Flow cytometry analysis of the percentage of Annexin V+ K562 cells (target cells) co-cultured for 4 h with naive or activated healthy human NK cells subjected to various treatments; the proportion of CD107a+ NK cells, IFN-γ+ NK cells and Granzyme B+ NK cells within the total population of these NK cells; the expression of NKG2D, CD160 and CD38 on these NK cell. n = 8. (c-d) Flow cytometry analysis of the percentage of Annexin V+ HL60 cells (target cells) co-cultured for 4 h with naive or activated healthy human NK cells subjected to various treatments; the proportion of CD107a+ NK cells, IFN-γ+ NK cells and Granzyme B+ NK cells within the total population of these NK cells; the expression of NKG2D, CD160 and CD38 on these NK cell. n = 8. (e-f) Flow cytometry analysis of the percentage of Annexin V+ MOLM-13 cells (target cells) co-cultured for 4 h with naive or activated healthy human NK cells subjected to various treatments; the proportion of CD107a+ NK cells, IFN-γ+ NK cells and Granzyme B+ NK cells within the total population of these NK cells; the expression of NKG2D, CD160 and CD38 on these NK cell. n = 8. Data in a-f were analyzed by one-way ANOVA with Tukey’s multiple comparisons test; means ± SD.
