Extended Data Fig. 7: The TCR diversity of Tex influences the antitumor efficacy.
From: CARD11 signaling regulates CD8+ T cell tumoricidal function
(a) The differentially expressed genes of CD8+ TIL cluster between WT and K215M mutant mice was indicated by different color bands based on scRNA-seq. Top 10 gene were shown. (b) Flow cytometry analysis of PD-1 and OVA Tetramer expression on CD8+ TILs in WT and Card11 mutant mice with B16-OVA tumors (left). The quantification of OVA Tetramer+ cells in WT and K215M mutant mice (n = 4) (right). (c) Donut plots show the clone frequency of Tex of CD8+ TILs from B16-OVA tumors. Each color in the inner ring represents a clone except the non-expanded clones (too many clones to split). Each color in the outer ring represents clones with the same amplification level. The number indicates the percentage of the most hyperexpanded clone in total Tex and the CDR3 sequences of this top clone were shown below. (d) The scheme of the TCR signal strength-dependent clone selection in tumors. Data represent mean ± SEM of n > 3 biological replicates. P values were determined using unpaired two-tailed Student’s t-tests.
