Extended Data Fig. 1: Characterization of complex III deficient CD8⁺ T cells from CD4-Cre RISP^fl/fl AOX^LSL mice.

Isolated CD8+ T cells from CD4-Cre RISP^fl/fl AOX^LSL mice of indicated genotypes were activated in vitro for 48 hr then collected for (a) ECAR or (b) TMRE and MitoTracker Green staining for flow cytometry. GSEA of naïve CD8 T cells from (c) RISP KO/WT genotypes or (d) RISP KO + AOX/WT genotypes. Expression profiles were compared against the Molecular Signatures Database (MSigDb) ‘Hallmark’ dataset v0.3 with the addition of published exhaustion gene expression profiles from Utzschneider NatImmuno 2020 (ref. ³⁷) and Wherry Immunity 2007 (ref. ³⁶), as well as the integrated stress response from Wong eLife 2019 (ref. ⁴⁴) (a gift from C. Sidrauski at the Calico Life Sciences). All data points represent individual mice as biological replicates. a. n = 2 WT, n = 2 KO, n = 2 KO + AOX; b. n = 4 WT, n = 4 KO, n = 4 KO + ; c-d. n = 4 WT, n = 5 KO, n = 3 KO + AOX. For a-b. data are means ± s.e.m, *P < 0.05 and statistical test in b. is two-way ANOVA with Tukey’s multiple comparisons test.