Extended Data Fig. 2: T cell defects in naïve CD4-Cre RISPfl/fl AOXLSL mice.
From: Mitochondrial respiration is necessary for CD8+ T cell proliferation and cell fate
Naïve CD4-Cre RISPfl/fl AOXLSL mice were used for direct ex vivo quantification by flow cytometry (a) of indicated populations from thymus, (b) percent of indicated naïve T cells that are apoptotic from pooled peripheral lymph nodes or (c) spleen (d) total indicated T cells from lymph nodes or spleen, (e) Percent of total T cell populations that express CD44 from lymph nodes or spleen, (f) percent of total T cell populations that are apoptotic by annexin V staining from lymph node or spleen. All data points represent individual mice as biological replicates, a. n = 20 WT, n = 13 KO, n = 10 KO + AOX; b-c. n = 8 WT, n = 7 KO, n = 6 KO + AOX; d. Lymph nodes n = 25 WT, n = 15 KO, n = 11 KO + AOX; Spleen n = 24 WT, n = 15 KO, n = 10 KO + AOX; e. Lymph nodes n = 21 WT, n = 15 KO, n = 13 KO + AOX; Spleen n = 21 WT, n = 14 KO, n = 12 KO + AOX; f. n = 8 WT, n = 7 KO, n = 6 KO + AOX. Data are means ± s.e.m, *P < 0.05, statistical tests are one-way ANOVA with Tukey’s multiple comparisons test, (d. CD4 LN *P = < 0.0376 WT/KO; CD8 LN *P = < 0.0001 WT/KO, *P = 0.0001 WT/KO + AOX; CD4+ Spleen *P = 0.0095 WT/KO, CD8+ Spleen *P = < 0.0001 WT/KO, *P = 0.0009 WT/KO + AOX; e. CD8+ LN *P = 0.0006 WT/KO; CD8+ Spleen *P = 0.163 KO/KO + AOX, *P = < 0.0001 WT/KO).
