Extended Data Fig. 2: An altered ILC3/ILC1 ratio increases susceptibility to C. rodentium infection.
a, Representation of relative abundances of specific bacteria at the phylum level between WT and DKO mice (n = 4 per group). b, Representation of relative abundance of specific bacteria at the genus level as in a. c, Boxplot representation of richness (Shannon’s alpha diversity index) for microbiome composition as in a. Boxes extend from the 25th to 75th percentiles, whiskers extend to 1.5 times the Interquartile Range (IQR), and the center line is the median. d, Principal coordinate analysis (PCoA) plot of bacterial beta diversity of 2314 unique taxa at the species level using Bray-Curtis dissimilarities. e, Representative hematoxylin and eosin (H&E) staining of gut sections from WT (n = 2) and DKO mice (n = 2) at steady state: scale bar, 100μm. f, Fecal lipocalin-2 (Lcn2) in WT (n = 4) and DKO (n = 3) mice at steady state measured by enzyme-linked immunosorbent assay (ELISA). g, Intestinal permeability measured by serum FITC-Dextran level and FITC-Dextran gMFI in DKO and WT littermates (n = 8 per group) at steady state. h, Body weight variation over time in WT (n = 5) and DKO mice (n = 4) infected with C. rodentium (2 × 109). i, C. rodentium fecal colony-forming units (c.f.u.) at indicated time point between 0 to 20 days post infection (dpi) as in h. Results are representative of two independent experiment (f, h, i) or pooled from two independent experiments (g). Statistics was performed using Wilcoxon rank sum exact test (c); unpaired two-tailed Student’s t-test (f, g); two-way ANOVA with Bonferroni’s multiple comparison test (h); Sidak’s multiple comparison test (i). Bars indicate means ± SEM.
