Extended Data Fig. 4: Role for IRF3 in STING-dependent inflammatory response in human and mice.
From: STING signals to NF-κB from late endolysosomal compartments using IRF3 as an adaptor
a, Representative images of C57BL/6-Wt, Irf3−/−, Ifnr1−/−, and Stinggt/gt mice after ionizing irradiation for 3 months. b, Levels of mRNA of Tnfa, Il6, and Cxcl10 in spleens from Wt, Irf3−/−, and Irf3R278Q/R278Q mice treated with LPS (5 mg/kg body weight) for 6 h. Mock-Wt and Mock- Irf3−/− (n = 4 mice), Mock- Irf3R278Q/R278Q (n = 6 mice), LPS-Wt (n = 5 mice), LPS- Irf3−/− and LPS- Irf3R278Q/R278Q (n = 6 mice). Results are presented as mean ± SD. P values were calculated using a two-sided, Brown-Forsythe ANOVA test followed by Dunnett’s T3 multiple comparisons test. c, Box plots of The Cancer Genome Atlas TCGA, RNA expression profiles in KIRC, CESC and LUSC. The highest and lowest 25% of STING expression were analyzed by comparing STING-high and STING-low groups. Statistical analysis was performed using a two-tailed Mann-Whitney test. The upper and lower ends of the boxes represent the upper and lower quartiles, and the horizontal line inside the box is the median of the dataset. The whiskers indicate the upper and lower extremes of the dataset.
