Extended Data Fig. 8: Gating strategy for flow cytometry analysis of human PBMCs.
From: STING signals to NF-κB from late endolysosomal compartments using IRF3 as an adaptor

a, PBMCs from three donors stimulated with mock or cGAMP were stained with antibodies against p65 pS536, STING pS358, and pS366, and analyzed by flow cytometry. The diagram illustrates the gating strategy to select the populations of interest. b, c, PBMCs from three SAVI patients and two healthy controls were stained with antibodies against p65 pS536, STING pS358, and pS366, and analyzed by flow cytometry. The diagram illustrates the gating strategy to select the populations of interest.