Fig. 4: NKp44+ ILC3s reside in lymphoid aggregates within the EM.
a, Representative image of lymphoid aggregates in the EM (top) and zoomed in (bottom). PanCK (white) identifies epithelial cells; DAPI (blue) identifies nuclei. b, Representative image of NKp44+ ILC3s at 10 µm. Images are separated by channels. Red arrows denote CD161+ ILC3s; white arrows denote CD161− ILC3s. c, Zoomed-in image of NKp44+ ILC3s in the aggregate near PanCK+ cells. d, NKp44− ILC3 in the EM. e, Quantification of NKp44+ and NKp44− ILC3s per area (log10 scale) in regions of interest (ROIs) that contained ILC3s: lymphoid aggregates (LA; n = 5 ROIs) and non-lymphoid aggregates (non-LA; n = 15 ROIs). Horizontal lines represent the median ± IQR. f, CD103−CD161+ ILC1s in the ECT. g, CD103+CD161− ILC1s in the END near the epithelium; n = 3 donors, with nine tissues total (EM, END and ECT for each). h, Number of CD161+, CD161− and CD103+ ILC1s per area (log10 scale) in ROIs containing ILCs: intraepithelial (n = 3 ROIs including the entirety of the epithelium from three patients), subepithelial (n = 5 ROIs) and deeper tissue (n = 7 ROIs). Each dot represents one ROI. ROIs from three patients were used. Horizontal lines represent the median ± IQR. Kruskal–Wallis test with Dunn’s post hoc test correction for multiple comparisons.
